Luciferase:Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model.
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged antiCD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged anti-CD40 was purchased from Abcam, Waltham, MA, USA.
Labeling:Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model.
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged antiCD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged anti-CD40 was purchased from Abcam, Waltham, MA, USA.
Cell Culture:Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model.
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged antiCD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged anti-CD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Stromal Estrogen Receptor-α Promotes Tumor Growth by Normalizing an Increased Angiogenesis
Article Snippet: .. Mouse Lewis lung carcinoma cell line (LL2, #CRL-2351), mouse breast tumor cells 4T1 (#CRL-2539), and mouse endothelial cell line derived by SV40 (#CRL-2181) were purchased and authenticated from American Type Culture Collection, routinely cultured as recommended by manufacturer and used from passages 3 to 8. .. B16K1, LL2, and 4T1 were last authenticated in February 2012 by Leibniz-Institut DSMZ GmbH.
Article Title: Design and evaluation of folate-modified liposomes for pulmonary administration in lung cancer therapy.
Article Snippet: .. LL2, a mouse Lewis lung carcinoma cell line (American Type Culture Collection, Manassas, VA, USA, CRL-1642TM), was cultured in Dulbecco’s modified Eagle medium (DMEM) containing penicillin (1 × 105 mU/mL) and streptomycin (0.1 mg/mL), supplemented with 10% FBS at 37 ◦C in a humidified atmosphere containing 5% CO2 and 95% air. .. KB cells were seeded on culture slides (Lab-Tek® II 8 chamber, Thermo Fisher Scientific, Massachusetts, USA) at a density of 5.0 × 104 cells/well and cultured in a humidified atmosphere containing 5% CO2 and 95% air at 37 ◦C for 24 h. After washing twice with HBSS (500 μL), C6-labelled FA-ssLip suspension (200 μL) dispersed in FBS-free RPMI 1640 was added and incubated for 2 h. The cells were then washed thrice with ice-cold HBSS (200 μL), followed by incubation with Hoechst 33342 solution (10 μg/mL) for 10 min. After washing twice with HBSS (200 μL), FBS-free RPMI 1640 (100 μL) was added, and the cells were observed using a confocal laser scanning microscope (LSM-700; Zeiss, Germany).
Modification:Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model.
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged antiCD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Aerosol Delivery of Hesperetin-Loaded Nanoparticles and Immunotherapy Increases Survival in a Murine Lung Cancer Model
Article Snippet: .. Luciferase gene labeled LL/2/Luc-2, from the mouse Lewis lung carcinoma cell line, was purchased from ATCC and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (GIBCO) with 10% FBS (Sigma Aldrich, Burlington, MA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). .. Red cherry fluorescent-tagged anti-CD40 was purchased from Abcam, Waltham, MA, USA.
Article Title: Design and evaluation of folate-modified liposomes for pulmonary administration in lung cancer therapy.
Article Snippet: .. LL2, a mouse Lewis lung carcinoma cell line (American Type Culture Collection, Manassas, VA, USA, CRL-1642TM), was cultured in Dulbecco’s modified Eagle medium (DMEM) containing penicillin (1 × 105 mU/mL) and streptomycin (0.1 mg/mL), supplemented with 10% FBS at 37 ◦C in a humidified atmosphere containing 5% CO2 and 95% air. .. KB cells were seeded on culture slides (Lab-Tek® II 8 chamber, Thermo Fisher Scientific, Massachusetts, USA) at a density of 5.0 × 104 cells/well and cultured in a humidified atmosphere containing 5% CO2 and 95% air at 37 ◦C for 24 h. After washing twice with HBSS (500 μL), C6-labelled FA-ssLip suspension (200 μL) dispersed in FBS-free RPMI 1640 was added and incubated for 2 h. The cells were then washed thrice with ice-cold HBSS (200 μL), followed by incubation with Hoechst 33342 solution (10 μg/mL) for 10 min. After washing twice with HBSS (200 μL), FBS-free RPMI 1640 (100 μL) was added, and the cells were observed using a confocal laser scanning microscope (LSM-700; Zeiss, Germany).
Derivative Assay:Article Title: Stromal Estrogen Receptor-α Promotes Tumor Growth by Normalizing an Increased Angiogenesis
Article Snippet: .. Mouse Lewis lung carcinoma cell line (LL2, #CRL-2351), mouse breast tumor cells 4T1 (#CRL-2539), and mouse endothelial cell line derived by SV40 (#CRL-2181) were purchased and authenticated from American Type Culture Collection, routinely cultured as recommended by manufacturer and used from passages 3 to 8. .. B16K1, LL2, and 4T1 were last authenticated in February 2012 by Leibniz-Institut DSMZ GmbH.
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